Rapid, DNA-based SCN egg counts

Protect Your Yield. Defend Your Margin. Know Your SCN.

NEMAGENE™ SCN delivers rapid, DNA-based soybean cyst nematode egg counts, tested from the same soil sample you already send in for fertility analysis, and validated against Iowa State's gold-standard method.

The economics

SCN Is a Tax on Every Bushel — One You Can Finally Measure.

Soybean cyst nematode causes up to 40% yield loss and more than $1.5 billion in annual damage to U.S. soybean production. It spreads quietly, builds resistance, and shows no symptoms until the yield, and the margin, are already gone.

Growers test soil fertility on millions of acres every year. SCN egg counting never kept pace. It's slow, labor-intensive, and expensive. NEMAGENE™ changes the math: the same soil sample, the same eggs/100 cc units, about half the cost, and a count you can act on before you buy seed or invest in an expensive nematicide-treated seed.

For Growers & Agronomists

No additional sampling, no specialty lab. Write "SCN" on your soil submittal form and your egg count arrives with your fertility results in the eggs/100 cc units you already use.

See how it works →

For Seed Brokers

NEMAGENE™ counts map to Iowa State's published SCN risk levels and show >90% correlation to Iowa State intervention guidance; a NEMAGENE™ number points to the same varietal and treatment decision the gold standard would.

See the decision logic →

For Soil Testing Labs

Add validated biological testing to your existing soil core workflow using a dried, ground sample, with under 3 hours to results, and up to 96 samples per run.

Bring NEMAGENE™ to your lab →
Gold standardValidated against the gold standard soil-displacement method — the industry benchmark.
>90%Correlation to Iowa State intervention guidance resulting in the same decision, faster.
PublicIndependent validation data openly available for review.
A platform, not a single test

SCN Is Just the Beginning.

Freyr Diagnostics is building the biological layer of routine soil testing: a multiplex-ready molecular platform designed for soil laboratories, starting with the most economically damaging pathogen in North American agriculture.

NEMAGENE™ SCN

Know Your Count Before You Buy Your Seed

NEMAGENE™ SCN delivers rapid, DNA-based soybean cyst nematode egg counts — no extra sampling, no separate lab, no waiting weeks for a microscope count.

Take the Test. Know Your Numbers.

SCN is the most damaging pathogen in North American soybean production — and one of the most manageable, if you measure it.

🫥

It hides

Fields can suffer substantial yield loss with no visible above-ground symptoms.

📈

It spreads

SCN keeps expanding its range and overcoming the common PI 88788 source of resistance.

🎯

It's manageable

Variety selection, seed treatments, and rotation all work — but only when you know your numbers.

Why NEMAGENE™ Is Different

No extra samples. No extra work.

SCN testing runs from your existing soil fertility sample — an additional analysis pulled from the routine core already on its way to the lab. No second pass. No separate cores.

Same units you've always used.

Egg counts are reported in eggs/100 cc of soil — identical to the traditional method — so your historical trend data stays useful.

Results with your fertility data.

Rapid qauntitative PCR delivers answers in under 3 hours, not days — alongside your nutrient report, in time for seed and management decisions.

About half the cost.

Streamlined molecular processing cuts the price roughly in half, making it practical to sample at grid density and actually see field variability.

More consistent year over year.

DNA-based quantification removes counting subjectivity, so trends reflect your field — not which technician was at the microscope.

Validated against the gold standard.

Proven statistically equivalent to Iowa State's soil-displacement egg count, with independent validation data publicly available.

For seed brokers & agronomists

The Same Decision as the Gold Standard.

Iowa State University publishes SCN egg-count categories that guide management: whether to rotate to a non-host crop, select a resistant or premium genetic variety, or add a nematicide seed treatment.

Because NEMAGENE™ reports in those same eggs/100 cc units, every count maps directly onto Iowa State's risk levels. Across the validation study, NEMAGENE™ levels showed greater than 90% correlation to Iowa State intervention guidance versus the gold-standard microscopy count. In practice: a grower acting on a NEMAGENE™ count reaches the same management decision they would have made on a gold-standard Iowa State test — only faster, cheaper, and often enough to track a field over time.

Low level

Lower intervention pressure — an option to plant a less expensive, less-resistant variety and protect margin.

Moderate level

Active management warranted — rotate resistance sources and consider a seed treatment.

High level

Justify the premium genetic variety and/or nematicide to defend the yield that's at risk.

From Sample to Decision

1

Sample once

Your standard soil fertility cores are all that's needed.

2

Check the box

Write "SCN" on your lab submittal form.

3

Get your count fast

SCN egg counts delivered with your soil nutrient results.

4

Decide with confidence

Counts map to Iowa State agronomic risk thresholds.

Yield per acre · Margin per acre

Stop Guessing. Start Deciding.

Today most SCN decisions are made on hope, hope that the variety holds, hope the seed treatment was worth it, hope last year's number still applies. When testing is cheap and frequent enough to run like routine fertility analysis, hope turns into a number that drives a better economic decision.

High count

Justify the premium genetic variety or a nematicide seed treatment and protect the yield that's at risk.

Low count

Skip the premium. Plant a less expensive variety and improve your margin with confidence.

Trending up

Adjust rotation before the population costs you a harvest.

Either way you're acting on data, not hoping — managing the two numbers that decide a soybean season: yield per acre and margin per acre.

Know your number — then build your plan. The SCN Coalition, the authority on SCN management, publishes field-tested intervention guidance for every risk level.

SCN Coalition Management Guide →
For laboratories

Biological Testing, Engineered for Your Workflow

NEMAGENE™ SCN was designed for commercial soil laboratories — not adapted from a research bench. It runs from the dried, ground soil sample already moving through your fertility line.

Built Around How Soil Labs Actually Operate

  • Same sample stream. Calibrated for a 20 cc aliquot from your standard dried, ground fertility sample.
  • Fast. Under 3 hours to results; ~20 minutes of hands-on time.
  • High throughput. Run up to 192 samples/day/technician.
  • Easy to staff. Cross-train existing teams; no nematology expertise required.
  • Familiar reporting. Output in eggs/100 cc, aligned to Iowa State risk categories.
  • Specific. Prep selects for eggs; PCR discriminates against look-alike species.

What's in the System

NEMAGENE™ Instrument

Cielo RT-PCR thermal cycler — 6-channel, 96-well, multiplex-ready open platform.

SCN Test Kit

Pre-aliquoted reagent tubes, 96 tests per kit. Two-channel assay: SCN target + internal control.

DNA Extraction Kit

Bead-beating tubes and diluent, 96 preps per kit.

Standard supporting equipment (centrifuge, vortex, cell disruptor, pipettes, freezer) — a full list and budget guidance are provided during onboarding.

Installation, Training & Support

We don't ship boxes — we stand up testing programs. Our three-day onboarding installs and qualifies your equipment, trains your team hands-on from 8 to 64+ samples per day, and tunes the workflow to your operation. Remote support continues after go-live.

It's the same lab-integration discipline our team used to deploy molecular diagnostics into hundreds of food and beverage laboratories worldwide.

Laboratory technician preparing NEMAGENE SCN samples at the bench

The Business Case

SCN testing demand is growing, driven by state soybean associations, university extension, and agronomists telling growers to "know your numbers."

  • Capture a new revenue line from samples already in-house
  • Differentiate against labs that outsource or decline biological testing
  • Serve grid-sampling customers at a price microscopy can't match
  • Position for the expanding Freyr panel — future assays on the same platform
The science

Validated Against the Gold Standard. Independently. Publicly

A new testing method earns adoption with data, not marketing. NEMAGENE™ was validated head-to-head against the Iowa State soil-displacement method and the full study is open for review.

How We Validated It

The independent validation was performed by Minnesota Valley Testing Laboratories (MVTL) and the Iowa State University, Tylka Nematology Laboratory. Eighteen bulk soil samples (>1 kg each; clay, loam, silt, and sand) were collected across southern Minnesota and processed by standard drying and grinding. Each sample was tested 4–6 times by both methods:

  • Reference (gold standard): traditional wet sieving and microscope egg counting using the Iowa State soil-displacement protocol — performed independently at both MVTL and Iowa State University.
  • NEMAGENE™ SCN: rapid PCR egg enumeration, performed at MVTL.

Average enumeration for each sample was compared by Deming regression to test for any statistical difference or bias between methods.

Soil core fertility sample being taken in the field
Soil Core Sampling. With NEMAGENE™ the same soil core fertility sample being taken can now be utilized for SCN counts as well.
Barcoded soil samples prepared and logged for analysis
Sample preparation. Barcoded soil samples are dried, ground, and logged, then a standardized 20 cc subsample is drawn from each for testing.

What the Data Showed

Across the expanded 16-sample set run at MVTL, the Deming regression returned a slope of 0.98 and a 95% CI of (0.70–1.38). In the eight-sample comparison against Iowa State, the slope was 0.93. A slope of 1 is a perfect match, and a confidence interval that includes 1 means there was no significant statistical difference observed between NEMAGENE™ and the gold-standard method in these studies.

Just as important for decision-making: when the counts are translated to Iowa State's management categories, NEMAGENE™ showed greater than 90% correlation to Iowa State intervention guidance — the same actionable answer as the gold standard.

Deming regression: NEMAGENE PCR count vs. microscopy count
Figure. NEMAGENE™ PCR egg count vs. gold-standard microscopy count across 16 bulk soil samples. Deming regression (red) tracks the identity line (dashed), slope 0.98.

Sample-by-Sample Results (vs. MVTL Microscopy)

Average egg counts per sample, NEMAGENE™ PCR vs. gold-standard microscopy.

Sample IDNEMAGENE™ PCR CountMicroscopy CountDifference
113,72419,646−5,922
37,3109,113−1,803
48,90511,183−2,279
56,64110,216−3,575
64,9705,720−750
73,5205,616−2,096
824,90324,163+739
913,28710,876+2,412
1015,53010,695+4,835
113,6612,487+1,174
122,2892,228+62
1310,1684,111+6,056
149,1057,188+1,917
155,0104,492+519
16335598−263
171,0442,750−1,706
182,6532,967−314
201,183814+369

Deming regression slope 0.98, 95% CI (0.7014, 1.3832). Slope of 1 = exact match. Source: NEMAGENE™ SCN validation study. Complete data sets for average values available upon request.

Read the Full Validation Study

📄 NEMAGENE™ SCN — Validation Whitepaper (PDF) Independent study by MVTL with Iowa State University Download PDF

Method Highlights

Quantitative RT-PCR with internal amplification control
Standardized 20 cc sample from dried, ground soil
Dynamic range <500 to >25,000 eggs / 100 cc
Selects for eggs — screens out adult & juvenile nematodes
Objective specificity for Heterodera glycines vs. look-alikes
Matrix-proven across hundreds of soil cores
"The results obtained with NEMAGENE™ were consistent with results of traditional microscope SCN egg counts conducted by the Tylka Nematology Lab at Iowa State University using shared soil samples." — Dr. Greg Tylka, Iowa State University
Our platform

A Long-Term Platform, Built for Soil Laboratories

Freyr Diagnostics is not a single-test company. We are building a scalable molecular diagnostics platform designed specifically for soil testing laboratories and agronomic networks.

Why Biology Isn't Routine — Yet

Physical and chemical soil testing became standard because it was validated, scalable, affordable, and aligned with how laboratories work. Biological testing never reached that standard — it stayed slow, specialized, and separate.

Freyr exists to change that. We're building the biological layer that will sit alongside nutrient and physical analysis as a standard component of soil testing. Not specialty. Not outsourced. Not research. Routine.

Barcoded soil cores prepared for analysis

Our Development Philosophy

  • Align assays to established agronomic risk thresholds
  • Validate against gold-standard reference methods
  • Engineer workflows compatible with routine soil core operations
  • Design multiplex-ready architecture to expand panels over time
  • Support laboratories with training, integration, and collaboration

SCN First. Then the Panel.

Soybean cyst nematode is our first assay because it's one of the most economically damaging and measurable biological risks in agriculture. The platform was never intended to stop there.

The NEMAGENE™ system is an open, 6-channel, multiplex-capable platform. In collaboration with soil laboratories and agronomy leaders, we're developing future assays targeting additional nematode species, soil-borne fungal pathogens, emerging crop threats, and biological risk indicators — all running on the same instrument and workflow labs install today.

Contact

Let's Put Biology in Your Soil Report

Growers, agronomists & seed brokers, want NEMAGENE™ at your soil lab? Tell us who you test with. Laboratories, are you ready to evaluate the platform? We'll walk you through validation data, workflow, economics, and onboarding.

Available Through Our Validation Partner

Minnesota Valley Testing Laboratories (MVTL)

New Ulm, MN
(800) 782-3557
www.mvtl.com

As the participating-lab network grows, this becomes a "Find a Lab" directory — pointing growers to the nearest lab offering NEMAGENE™.